STart Coagulation Analyzer

A brief introduction to a coagulation analyzer, which performs imunoassay, clotting, and chromogenic assays.

A Transcription of STart Coagulation Analyzer

Welcome back to BioMedBuddy.

Today, we are going to go through a quick checkout of the STart Coag Analyzer by Diagnostica Stago. This is an extremely reliable unit. By the way, everything I tell you here is pretty much true for the ST4 also, which is also a very reliable unit. There's no big benefit from my viewpoint or big difference between the STart and the ST4. Regardless of that, first of all let's talk about the accessories you should get with it. (Narrator displays accessories, :45) You should get a pipettor. Yes, you can run the unit without a pipettor. Sure, it makes it a lot more clumsy to use it. This is an ST4 pipettor. They are interchangeable. This is a really solid pipettor.

This is a STart pipettor. I guess I don't care for it just because it like plastic and just feels pretty flimsy, but hey. Ball dispenser - of course, hopefully you get some balls with it. It's not necessary to check out the unit, but surely if you do a complete checkout you'll have to have the balls. With a little luck to do a complete checkout you're going to need the cuvette holders.

The first check we are going to make is that we are going to simply turn it on. (Narrator flips switch on back right side of machine, 1:32) And this is the first time this has been checked out, by the way. I just got this from eBay. Of course, I'm capable of repairing these units. I'm the reason for this demo here, so that you get one that's already working.

It does a serial line test, it did a ram test, it printed out the self-test, okay. (Narrator points to screen and printout, 1:59) If gets to this point for the most part you can feel fairly confident that this instrument is going to work. But it's not the end of the test I'm going to recommend. I'm going to hit just by reading this display I'm going to hit any key and hit enter. I'm going to hit, “4”. (Narrator presses a series of numbers on the keypad, 2:20-3:49)

This is the system check, enter. I want to do, “2”, the diagnostic check, “Enter”. “Enter”, to run self-check.

This is for the keyboard test. It wants me to press “1”, then “2”, then “3”, then “4”, then the “pipettor”. “1” key, “2” key, “3” key, “Enter” key, “4”, “5”, “6”, “Escape”, “7”, “8”, “9”, “Paper Advance”, “arrow key to the left”, “0”, “decimal point”, “arrow key to the right”, press a key to continue – it says keyboard test error. Somewhere I pressed either the wrong keyboard, the wrong button, or I'm not fast enough, or there's a problem with the keyboard. But we're going to continue. I really didn't see the problem, but we're going to continue.

Now it's going to do a ram test. Ram okay. Press continue. (Inaudible, 3:32) test. Press a key to continue. We're checking now your display. See all the dots are present. “Enter”. Again, all the dots are present. Good display. Press a key to continue.

Pipette test. They want you to press your pipette. Pipette test okay. Serial 9 test, this takes a second. That just checked out the pipettor, the electronics of the pipettor is good. It does not check out the repeatability of the pipettor, which if you give me a call or an email I can tell you who can check out the, who would do the pipettor test.

In fact, actually I could do that right now. (Narrator displays pipettor, 4:21) Pipettor repair services, this has just been certified by them this year, in January of (Inaudible, 4:21). End of self test. We're all good.

Now, just so you know, if I hit, “2” and hit, “Enter” again, and let's see, I think I have to hit, “0”.(Machine screen is displayed, 4:41) I get to another test which is pretty much
service orientated and I am not going to get involved with this program. You can cause problems if you do. I'm just mentioning that it's there so if you're using this video to do more troubleshooting at least you're aware. Probably going to cause yourself more problems than its worth, but anyways. It's how you calibrate the temperature of the channels and some of the measurement wells. We're not going to do that.

All right, hit, “Escape”. We'll get back out of that. Back to the Diagnostica Stago main menu. We're going to go for a second here to, and I'm cheating here and I know this. Just want to check out the measurement station without having any reagents. We're going to go into test mode. This is just a general check. (Narrator presses a series of numbers on keypad, 5:42-6:36)

“1”, “Enter”. Hitting the right button helps. Now we're just going to just, for grins and giggles, is we're going to go, “2”, “Enter” for the PT. Patient ID for one we're going to use, “1”, “2”, “Enter”. Two, we're going to do, “4”, “5”, “Enter”, “7”, “8”, “Enter”, “.0”, “Enter”. That doesn't look right. Five, we're going to go, “6”, “Enter”, “6”, “7”, “Enter”. I'm trying to figure out what key isn't working here - I don't see; they're all working.
“8” and we'll go, “Enter”. Okay, and that's all we want to do.

Now, we are simply going to cheat the system. (Places cuvette in far left opening, 6:47) That's why I said I hope you have cuvettes. I am going to put just a little distilled water in it and again, this is just a check that you can do, just to check it and make sure all my measurement channels are essentially working. (Pours distilled water in cuvette, 7:01) So it gives you kind of an understanding of how it used to work. We're going to put it in incubation. We're going to put a bb in each one of them. (Drops bb in each opening, 7:14) By the way, if you miss a bb in it, this will give you all sorts of little headaches.

Okay, and we're going to start our timer. (Narrator presses buttons on bottom left side of machine, 7:32-7:58) So, we're going to start our timer and just make sure that it works. While we're doing this, when you get up to 5 or 6 seconds, you can check your other timers. More than likely at this point they're going to work, but I'm going through the motions. And you'll see all your timers and that's just counting up by seconds so you'll know how long you've been in the incubation section.

You also can see your temperatures, 37 degrees, and then after this we're going to move all of our samples so we know all our timers are working. We're going to move all our samples, making sure that all the bb’s are there, all of them right over here. (Narrator moves cuvette to far right opening, 8:11) We're going to take our pipettor and we're going to start our pipetting.(Narrator lifts pipette, presses the button beneath loaded cuvette, and points to screen, 8:23) I'm going to actually use one of the timers, just so you know. (Narrator presses a series of buttons, 8:26-8:58) I'm going to hit 57, 58, 59, 60, let's do 70, 66, 67, 68, 69, 70, 1.

All right, as you can see I just started all my tests. All my tests are ready. With a little luck that should be all of them. And of course I started them, when I hit the button I started this timer for making that measurement. (Camera pans in on screen, 9:15)

So, the first one is going to be, let's just say, 0, the next one's going to be started at, 2 seconds, the next started at, 3 seconds, the next started at maybe, 5 seconds, the next at, 6 seconds. They all started about a second or so away from each other. Like I said, probably 1 or 2 and we're going to find out in a second because I'm trying to wait for a
little bit so that we get a realistic reading somewhere around the 35. So, if you wait 35, 40 seconds. And then what you ought to do is you can simply pull the unit out. (Narrator removes cuvette, 9:47)

And as you can see, we got test results. (Camera pans in on screen, 9:54) And you'll be able to see the 67.4, that should be essentially less time counting downward. The first one in there's going to take the most, the second one a little less and so forth.

Now, if you wanted to get a repeatability you could actually put in one at a time and time each one of them. And pull them out so that you'll get the same number. (Narrator pulls paper, 10:26) You have to be pretty accurate at that, but hopefully you get the idea. For the most part, I will tell you, this instrument is going to work for samples. Thank you very much for attending BioMedBuddy training session for the STart unit. This is a quick check up designed for somebody buying a STart or an ST4 on eBay. Simply to make sure you get a good unit. Thank you.