Troubleshooting the STA Compact Service Part 3

Tutorial on determining the source of an issue for the STA Compact, Part 3: Mapping. See also: parts 1, 2, 4, and 5.

Troubleshooting the STA Compact – Service Part 3

Welcome back to BiomedBuddy training on the STA compact. By chance, you just looked at the endurance you saw I had a bent needle. We are going to go through mapping in order to try and correct that. Okay, I backed out, I'm still in the service. I'm going to go into the robotics.(Screen change, :19) Robotics down to mapping. (Screen change, :23)We're going to map on number one, not much to arm number two. (Pop-up displayed, :30)

In fact, I can't say general for the most part you never have to map number two because it's a stationary arm; there is very little that can go wrong with it. (Camera pans to STA Compact, :43) Or if something does go wrong you should ask yourself why, because for the most part, you should never have to map number two unless you change some component like maybe the back motor.

We are talking about arm number one, washing wells first thing. And we're going to have to move back and forth a lot here, so I'm sorry. (Camera pans to computer, :59) Arm number one, okay, now let's go into adjustment, F2 for adjustment. (Pop-up displayed, 1:03) (Camera pans to STA Compact, 1:08) All right number two just went, excuse me, arm number one just over the wash wells.

We've got a little tool here it's a mapping tool. (Narrator shows mapping tool, 1:16) I love this thing.(Narrator places mapping tool in machine and adjusts needles, 1:24-1:59) It's one of the nicer things Stago came up with, and what it allows me to do isput the needles in the dead center of my wash wells.

Now you watch me if these were new needles, in theory that should go right in. But since they're not new needles I'm going to make some adjustments. Because these are just somehow, some reason, have gotten bent and I'm making the adjustments.

So that needle goes straight down into wash well number one. Okay, goes right in. I'm going to put my hand back, and there's a little wheel. And you want you to be a little careful with this. (Narrator manually lowers needles with wheel on the back of the arm, 2:06-5:37) Meaning that you don't want to press too hard on the wheel, but I'm going to move it up so that you can see that the wheel is there. And that you can do it, and you also, some manhandle the arms.

Okay, now you can tell that is going right into the center. I'm going to do the same for each needle. And needle number three is the interesting one, but regardless of that. Okay and it goes right back in. Now that's fine, that just means that my needle is centered.

Now we're going to go to needle number two. You don't want to move the arm. You should have already, by the way, opened up the draw at least once and closed it. Let the instrument do it because that would position it, not critical, but that's what you should do. I just know because I went through endurance that the doors been opened and closed. And you see me bend it back a little. We're not talking about an awful lot we're just talking about minor adjustments. Because there should be no kinks or anything in this needle.

And now the arm number three. All right let's put it down to my hand. See how that's off quite a bit, and that's because it was bent and we're not going to try to replace that needle. This is what I mean by saving yourself some needles because there's nothing wrong with this needle. You can go back and check it out later, but the odds are there's nothing wrong with that needle. But it is a little bent now. And like I said if you saw me doing the endurance you would know why it's bent.

Okay for the most part that goes down right into the center. By the way, a lot of times what I do is when I first come to an instrument I take them, and I put them all the way down. The reason that is, is you can tell if a needles has been bent because, in theory, this part is all locked in. This is always even from one mapping to another should always be correct, assuming it was mapped correctly the last time. I'm going to raise my needles up, taking the mapping cuvette out. (Narrator removes mapping cuvette, 4:12)

All right now I'm going to lower all three needles into the wash well. I'm going to go all the way down with all my needles. And that is not where we're going to leave them. I'm all the way down. Now you've heard me talk about BB's getting caught in the wash well, and I think this is the main reason you do this. Is I'm going to raise, they're all the way down, I'm going to raise each one just a tiny little bit, like a BB, BB distance,
just a little. This is what determines how low, these needles go into the wash well.

There's the bottom, here's how high I'm raising it. Just a little distance. Again, this is what determines the mapping of the wash well determines that it's where the needles are going to go in the wash well, meaning center and how deep Z-movement how far down there it's going to go. You can see from this you can't really overdo it; if you every get the needle crashing into the wash well there's either a BB in it or some other problem like the gear being loose or something along that lines.

All right we're good. Now I'm going to go back over to the screen and I'm going to hit F10, (Camera pans to computer, 5:44) >as you can see it actually says, Z-movement here. F10 to validate, (Pop-up displayed, 5:50) and back over to my screen it's all came back up and now it's zero. (Camera pans to STA Compact, 5:54) And now we're going to go down to F4, (Camera pans to computer, 5:59) F4 is really in drawer number one, it's not really that critical, (Screen change, 6:06) but we're going to hit F4 anyway just to do it and hit F2 to make the adjustment. (Pop-up displayed, 6:11) (Camera pans to STA Compact, 6:18)6) o computer, 5:59) 4) 4:12)-5:37)